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USMLE Step 1 Bits & Pieces High yield short focused points, monographs, charts, illustrations, tables, and other stuff related to the USMLE Step 1 Exam.


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  #1  
Old 07-01-2011
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Bacteria High Yield Microbiology Growth Media/Agars for USMLE Step 1

MacConkey Agar:

High Yield Microbiology Growth Media/Agars for USMLE Step 1-macconkey-agar.jpg
click image to enlarge

- Contains bile salts
- Inhibit G(+) bacteria
Lactose fermenters turn the colonies pink.
Colonies of the non-lactose fermenters are colourless.


Eosin methylene blue:

High Yield Microbiology Growth Media/Agars for USMLE Step 1-emb2.jpg
click image to enlarge
High Yield Microbiology Growth Media/Agars for USMLE Step 1-emb1.jpg
click image to enlarge

- Inhibit G(+) bacteria
Bacteria that ferment lactose, especially the coliform bacterium Escherichia coli, appear as colonies with green metallic sheen or blue–black to brown color. Bacteria that do not ferment lactose appear as colorless or transparent light purple colonies


Hektoen Enteric Agar:

High Yield Microbiology Growth Media/Agars for USMLE Step 1-hektoen2.jpg
click image to enlarge

Medium designed to isolate and differentiate members of the species Salmonella and Shigella from other Enterobacteriaceae.
- Bile salts and the dyes bromthymol blue and acid fuchsin inihibit the growth of most G(+) organisms.
- Lactose, sucrose, and salicin provide fermentable carbohydrates to encourage the growth and differentiation of enterics.
- Sodium thiosulfate provides a source of sulfur. Ferric ammonium citrate also allows the visualiztion of hydrogen sulfide production by reacting with hydrogen sulfide gas to form a black precipitate.
Salmonella typhi : Colorless colonies, black centers.


Thiosulfate Citrate Bile Salt Sucrose agar plate (TCBS)

High Yield Microbiology Growth Media/Agars for USMLE Step 1-tcbs.jpg
click image to enlarge

- For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical specimens and in epidemiological investigations.
Vibrio cholerae : Growth (yellow colonies)
Vibrio parahaemolyticus : Growth (green colonies)
Staphylococcus aureus : No growth


Chocolate Agar:

High Yield Microbiology Growth Media/Agars for USMLE Step 1-chocagar.jpg
click image to enlarge

Is prepared by adding sheep blood to an enriched agar base when the temperature of the medium is high enough to lyse the red cell without inactivating the NAD (V factor) and hematin (X factor) in the blood lysate (about 80C)


Thayer Martin VCN:

High Yield Microbiology Growth Media/Agars for USMLE Step 1-thayer-martin.jpg
click image to enlarge

- with 5% chocolate sheep blood plus
- Vancomycin, which is able to kill most Gram-positive organisms
- Colistin, which is added to kill most Gram-negative organisms except Neisseria
- Nystatin, which can kill most fungi.


Pseudomonas aeruginosa (extra)

High Yield Microbiology Growth Media/Agars for USMLE Step 1-pseudomona.jpg
click image to enlarge

Blood Agar: greenish-metallic appearing colonies (production of the pigments pyoverdin and pyocyanin)
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  #2  
Old 07-01-2011
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nice post!
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bebix, u rock!
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Old 07-02-2011
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Default yes babix

You rock ...:d
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thank u
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Great ! thanks
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Old 07-02-2011
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nice post !!!
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Old 07-02-2011
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thank u
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  #9  
Old 12-13-2012
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Quote:
Originally Posted by bebix View Post
macconkey agar:

Attachment 1650
click image to enlarge

- contains bile salts
- inhibit g(+) bacteria
lactose fermenters turn the colonies pink.
colonies of the non-lactose fermenters are colourless.


eosin methylene blue:

Attachment 1647
click image to enlarge
Attachment 1648
click image to enlarge

- inhibit g(+) bacteria
bacteria that ferment lactose, especially the coliform bacterium escherichia coli, appear as colonies with green metallic sheen or blue–black to brown color. Bacteria that do not ferment lactose appear as colorless or transparent light purple colonies


hektoen enteric agar:

Attachment 1649
click image to enlarge

medium designed to isolate and differentiate members of the species salmonella and shigella from other enterobacteriaceae.
- bile salts and the dyes bromthymol blue and acid fuchsin inihibit the growth of most g(+) organisms.
- lactose, sucrose, and salicin provide fermentable carbohydrates to encourage the growth and differentiation of enterics.
- sodium thiosulfate provides a source of sulfur. Ferric ammonium citrate also allows the visualiztion of hydrogen sulfide production by reacting with hydrogen sulfide gas to form a black precipitate.
salmonella typhi : Colorless colonies, black centers.


thiosulfate citrate bile salt sucrose agar plate (tcbs)

Attachment 1651
click image to enlarge

- for the selective isolation of vibrio cholerae and vibrio parahaemolyticus from a variety of clinical specimens and in epidemiological investigations.
vibrio cholerae : Growth (yellow colonies)
vibrio parahaemolyticus : Growth (green colonies)
staphylococcus aureus : No growth


chocolate agar:

Attachment 1652
click image to enlarge

is prepared by adding sheep blood to an enriched agar base when the temperature of the medium is high enough to lyse the red cell without inactivating the nad (v factor) and hematin (x factor) in the blood lysate (about 80c)


thayer martin vcn:

Attachment 1653
click image to enlarge

- with 5% chocolate sheep blood plus
- vancomycin, which is able to kill most gram-positive organisms
- colistin, which is added to kill most gram-negative organisms except neisseria
- nystatin, which can kill most fungi.


pseudomonas aeruginosa (extra)

Attachment 1654
click image to enlarge

blood agar: Greenish-metallic appearing colonies (production of the pigments pyoverdin and pyocyanin)
thank you so much, i was looking this on google!
Good luck!
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  #10  
Old 01-01-2015
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what about buffered charcoal yeast agar??
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