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  #1  
Old 01-09-2010
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Default DNA technique question

In a laboratory a standardized cDNA library and cloned DNA from rats were both subjected to specific DNA binding proteins. DNAse was then added and the fragmentation pattern of the samples were studied.
What do we call this procedure?

a- Southern blotting
b- Southwestern blotting
c- Northewestern blotting
d- DNA footprinting
e- Polymerase Chain Reaction
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Old 01-09-2010
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Southwestern blot. Detecting proteins is western blot. Detecting DNA is Southern blot. Southwestern is a combination.
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Old 01-09-2010
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the description does not go with southwestern blot
I think this is DNA footprinting
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Old 01-10-2010
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Quote:
Originally Posted by 473-syndrome View Post
the description does not go with southwestern blot
I think this is DNA footprinting
I totally agree, but is it commonly asked in the exam?
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Old 01-10-2010
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Quote:
Originally Posted by DrSeddik View Post
I totally agree, but is it commonly asked in the exam?
The idea here is to know that all other options are incorrect and therefore you should choose DNA footprinting even if you don't know anything about it. Yes, such "exclusion" questions can be seen in the exam.
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Old 01-10-2010
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Please explain why this is not Southwestern blotting.
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Old 01-10-2010
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Quote:
Originally Posted by sak2114 View Post
Please explain why this is not Southwestern blotting.
In southwestern blot you spread proteins on filter paper then you add labeled DNA probe to see which protein bind to it.
This is not what's described in this question. What's described is a typical DNA footprinting technique where two samples of DNA are analyzed by adding DNA binding proteins then subjected to DNAase then you analyze the fragmentation pattern (DNA binding proteins protect DNA from lysis by DNAases). If the fragmentation pattern is identical then the two samples must be coming from the same or closely related species or individuals.

Also look at this thread for further details
Northern vs Southern vs Western Blot - HELP!
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Old 01-10-2010
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OMG, this is really scary
You guys all figured out the answer, but I did not
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