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Old 08-15-2011
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Bacteria Cystic Fibrosis Microorganism of major concern!

A 17-year-old girl with cystic fibrosis has a slight increase in her frequent cough and production of mucoid sputum. A sputum specimen is obtained and plated on routine culture media. The predominant growths are gram-negative bacilli that form very mucoid colonies after 48 hours of incubation. These bacilli are oxidase-positive, grow at 42 °C, and have a grape-like odor.The sputum also is plated on a colistin-containing agar. After 72 hours of incubation, the colistin-containing agar grows gram-negative bacilli that are oxidase-positive but are otherwise difficult to identify. This microorganism is of major concern. It is sent to a reference laboratory so that molecular methods can be used to identify or rule out which of the following?

A. Pseudomonas aeruginosa
B. Burkholderia cepacia
C. Haemophilus influenzae
D. Pseudomonas putida
E. Burkholderia pseudomallei
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A.pseudonomas aeruginosa
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A. Pseudomonas aeruginosa
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so easy its A sureeeee .. psedomonas aerognisa can cause pnemonia in systic fibrosis patients ... and presents with fruity odour ..clue here oxidase positive grape like odour ... and gram negative
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pseudomonas oxidase + gram - fruity odour it favours it
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Quote:
Originally Posted by akua View Post
A.pseudonomas aeruginosa
yup its aaaaaaa
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Old 08-15-2011
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ok the ans is burkholderia cepeciae
the organism is gm-ve oxidase positive it causes pneumonia in immunocompromised pt n in CF
the key point is it is resistant to colistin while p.aeruginosa is sensitive
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Default @Qurat21 awesome job dude !!!!

Quote:
Originally Posted by qurat21 View Post
ok the ans is burkholderia cepeciae
the organism is gm-ve oxidase positive it causes pneumonia in immunocompromised pt n in CF
the key point is it is resistant to colistin while p.aeruginosa is sensitive


b)burkholderia cepeciae


check this out http://en.wikipedia.org/wiki/Burkholderia_cepacia
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Old 08-15-2011
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excellent question mate.... didnt know about the colistin concept.. thanx
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Default ans:

Quote:
Originally Posted by drgsarunprasath View Post
A 17-year-old girl with cystic fibrosis has a slight increase in her frequent cough and production of mucoid sputum. A sputum specimen is obtained and plated on routine culture media. The predominant growths are gram-negative bacilli that form very mucoid colonies after 48 hours of incubation. These bacilli are oxidase-positive, grow at 42 °C, and have a grape-like odor.The sputum also is plated on a colistin-containing agar. After 72 hours of incubation, the colistin-containing agar grows gram-negative bacilli that are oxidase-positive but are otherwise difficult to identify. This microorganism is of major concern. It is sent to a reference laboratory so that molecular methods can be used to identify or rule out which of the following?

A. Pseudomonas aeruginosa
B. Burkholderia cepacia
C. Haemophilus influenzae
D. Pseudomonas putida
E. Burkholderia pseudomallei

pseudomonas aeruginosa.............
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Old 08-15-2011
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A Pseudomonas aeruginosa
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