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Old 09-19-2011
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Question Bug bite and immunoblotting!

A 7-year-old boy presents to the hospital with ptosis and drooping on the left side of his face for 2 days. He also complains of pain and swelling in his right knee, which began about a week ago. The boy's mother reports no significant past medical history, and states that the boy has been spending a lot of time playing outside. She also recalls that the boy had a “bug bite” several weeks ago that produced a ring-shaped rash. Which of the following techniques will have the highest specificity for detecting the antibodies for the pathogen responsible for this patient’s illness?

A. Enzyme-linked immunosorbent assay (ELISA)
B. Polymerase chain reaction (PCR)
C. Restriction fragment length polymorphisms (RFLPs)
D. Southern blotting
E. Western blotting

Source: Kaplan Qbank
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Old 09-19-2011
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lyme disease with facial nerve pulsy(bulls pulsy)

i think
E. Western blotting
The Western blot is more specific for B. burgdorferi than the ELISA because it identifies antigens individually

http://www.uptodate.com/contents/pat...-and-diagnosis
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Old 09-19-2011
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For higher specificity always go with western blot
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Old 09-23-2011
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Western Blot
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Old 09-24-2011
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This patient demonstrates a late-phase presentation of Lyme disease, which is caused by the spirocheteBorrelia burgdorferi and is spread by the bite of infected Ixodes ticks. The most specific technique to detect the reactive antibodies is via Western blotting, which can confirm reactivity as well as molecular weight.
ELISA (choice A) can also detect the antibodies, but since they are not separated by molecular weight, it is less specific.
PCR (choice B), RFLP (choice C), and Southern blotting (choice D) are all methods that detect nucleic acids, not proteins.
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