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Old 09-19-2011
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Help Q about genetic techniques

Q:A 28y woman and a 25y man present fore genetic counseling. Both are white and have one sibling affected with cystic fibrosis. The most appropriate method to assess the risk of transmitting cystic fibrosis to a potential child would be:

A. biochemical testing
B. FISH
D. PCR
E. Western blot analysis



The correct answer is D, but I think all the 4 answers work. A and E could examine the defect protein, B and D could examine the defect DNA. Need some help here, thank you!!!
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Old 09-20-2011
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Default pcr

pcr?
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Old 09-21-2011
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I've got a nice answer from another forum, see here:

http://www.prep4usmle.com/comments.p...=last#lastpost
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Old 09-21-2011
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Yes, answer is PCR. Even on UW/Qbank Kaplan and usmle RX keep telling you PCR...
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Old 09-21-2011
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Quote:
Originally Posted by ZoeZ View Post
Q:A 28y woman and a 25y man present fore genetic counseling. Both are white and have one sibling affected with cystic fibrosis. The most appropriate method to assess the risk of transmitting cystic fibrosis to a potential child would be:

A. biochemical testing
B. FISH
D. PCR
E. Western blot analysis



The correct answer is D, but I think all the 4 answers work. A and E could examine the defect protein, B and D could examine the defect DNA. Need some help here, thank you!!!

It could be only PCR .
It detects defective gene.
Other options cant true in any sense.
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