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  #1  
Old 03-10-2010
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Question Thymidine Incorporation Values?

A 40 year old man is scheduled for a kidney allograft. His lymphocytes (with thymidine incorporation value of 425/cpm) are examined by the mixed lymphocytes reaction with killed stimulator cells from himself and from each of 5 prospective donors. A donor with which of the following thymidine incorporation value (cpm) will be most compatible?

A- 312
B- 1532
C- 2510
D- 15,390
E- 27,482
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  #2  
Old 03-10-2010
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What is this? Never heard of it
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Old 03-15-2010
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Possible A, 'cos that which is lower than his.

Last edited by bianca; 03-15-2010 at 12:02 AM. Reason: typing error
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  #4  
Old 03-15-2010
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Default What's Thymidine Incorporation Value?

Quote:
Originally Posted by bianca View Post
Possible A, 'cos that which is lower than his.
Yea, but do you have any idea what's thymidine incorporation value? I Googled it and found nothing
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Old 04-11-2010
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Quote:
Originally Posted by Homer88 View Post
Yea, but do you have any idea what's thymidine incorporation value? I Googled it and found nothing

Thymidine incorporation value is an indicator of immunogenicity. The higher the uptake, the more the pt's wbc's are gobbling up the transplant patient's thymidine.
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  #6  
Old 04-12-2010
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Quote:
Originally Posted by ClockworkDoc View Post
Thymidine incorporation value is an indicator of immunogenicity. The higher the uptake, the more the pt's wbc's are gobbling up the transplant patient's thymidine.
oh so does that mean we have to a thymidine incorporation value that is higher than the ptt's? what's the answer then? how much higher? or is it that the ptt's WBCs are MEANT to gobble up the donor's WBCs?
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  #7  
Old 07-03-2010
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Classically, T cell alloreactivity has been measured in vitro using 3H-thymidine incorporation to assess T cell proliferation in the mixed lymphocyte reaction (MLR)...lower Tcell reaction, better tolerance no?
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Old 07-05-2010
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Do you guys know where this question came from? I'm wondering if there are any other similar questions...
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Old 12-02-2010
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Default mixed lymphocyte reaction (MLR)

In this test, "stimulator" lymphocytes from a potential donor are first killed by irradiation and then mixed with live "responder" lymphocytes from the recipient; the mixture is incubated in cell culture to permit DNA synthesis, which is measured by incorporation of tritiated thymidine. The greater the amount of DNA synthesis in the responder cells, the more foreign are the class II MHC proteins of the donor cells. A large amount of DNA synthesis indicates an unsatisfactory "match"; i.e., donor and recipient class II (HLA-D) MHC proteins are not similar, and the graft is likely to be rejected. The best donor is, therefore, the person whose cells stimulated the incorporation of the least amount of tritiated thymidine in the recipient cells.
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Old 12-03-2010
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Hey guys! be careful; answer is b. his baseline line thymidine incorporation value is 425/cpm. if we mix dead donor cells, it is going to increase, not decrease. if the donor was a perfect match, then the answer would have been 425/cpm. cells don't respond. without a perfect match, recipient cells has to show some reaction and a value that is higher than baseline.
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  #11  
Old 01-06-2011
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Quote:
Originally Posted by drahmednawaz View Post
Hey guys! be careful; answer is b. his baseline line thymidine incorporation value is 425/cpm. if we mix dead donor cells, it is going to increase, not decrease. if the donor was a perfect match, then the answer would have been 425/cpm. cells don't respond. without a perfect match, recipient cells has to show some reaction and a value that is higher than baseline.

i got this right and the answer was A ! they mention clearly about the donor with the thymidine value - hence they are referring to the opposite reaction.
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  #12  
Old 01-06-2011
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Quote:
Originally Posted by Seetal View Post
oh so does that mean we have to a thymidine incorporation value that is higher than the ptt's? what's the answer then? how much higher? or is it that the ptt's WBCs are MEANT to gobble up the donor's WBCs?
Well i wonder if they are gobbling up the thymidine from the other patient :P might be in the media... one needs DNAases to break down DNA into thymidine.. i dont know why lymphocytes would need that since they arent in the business of lysis like neutrophils .. or staph aureus.

dTTPs are provided in the medium to measure proliferation.(please verify)
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Old 01-06-2011
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i chose A cause i thought that immunosuprresants would make the graph more acceptable cause immosupresant decrease production and activity of immunlogical cells which would lower the value for the thymidine incorporation level which would then therefore decrease the chance of an autoreactive reaction... i think my thinking is wrong, or am i just not going deep enough.. this q confused me a little. meh idk hahaha

edit 2.. yup my thinking is wrong after reading the posts here lol.. ugh
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  #14  
Old 01-24-2011
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I put B and got it wrong. Likely answer is A.
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Old 05-03-2011
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Refer to:


http://books.google.com/books?id=EJX...tation&f=false

you will found your answer....
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  #16  
Old 05-03-2011
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This test is given in detail in Kaplan immunology..The answer is A..Thymidine incorporation value measures the proliferation of T cells in response to antigen which is MHC class II in this case.
As described above by some one this test requires an antigen ..For this we can use the recipients Tcells ..Now the test is as follows....
Recipients T Cells are irradiated to prevent it from proliferation.They will provide for the MHC antigens. When the donors Tcell are mixed with recipients irradiated T cells and if donors cell recognize it as foreign they will proliferate using up thymidine..Hence when the scores are low it means a better chance to match for tissue grafts..The test is done both ways..i.e donors cells are also irradiated and mixed with recipients Tcell to rule out mis match..
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  #17  
Old 11-16-2011
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Default thymidine incorporation value??

what is thymidine incorporation value that is use in organ transplantation ??
any one knows??
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  #18  
Old 11-16-2011
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Hey Check out this link: Thymidine Incorporation Values?

I think its a measurment about how reactive the t-lymphos of the host are going to be against the graft and a low value should be a good sign. Hope it helps!
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  #19  
Old 11-16-2011
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Quote:
Originally Posted by Glomerulus View Post
Hey Check out this link: Thymidine Incorporation Values?

I think its a measurment about how reactive the t-lymphos of the host are going to be against the graft and a low value should be a good sign. Hope it helps!
thank you
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  #20  
Old 11-16-2011
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No problem Was this in the NBME 7? Which NBME have u taken so far? Which one did u find the most difficult?
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Old 11-16-2011
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Quote:
Originally Posted by Glomerulus View Post
No problem Was this in the NBME 7? Which NBME have u taken so far? Which one did u find the most difficult?
yes it is NBME 7
i am doing them offline
i did 12 and half 7
12 is easy
7 is more tough

my exam after a month
how about u?
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  #22  
Old 11-16-2011
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My exam is in 2 weeks Havent taken an NBME yet, but I will take one this weekend. NERVOUS I am hesitating btw 7 and 12. Will see. Have u taken the UWSA?
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  #23  
Old 11-16-2011
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Quote:
Originally Posted by Glomerulus View Post
My exam is in 2 weeks Havent taken an NBME yet, but I will take one this weekend. NERVOUS I am hesitating btw 7 and 12. Will see. Have u taken the UWSA?
no i didnt take them i advice u to take 7 coz 12 was really easy and u dont want to overestimate yourself
actually i got 185 right out of 200 in 12 and i wonder if that is good
but when i was solving 7 it seems a lil bit harder

dont freak out my friend you r going to win this battle just relax and try to be good with your FA if u want any help we can talk on skype or emails
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Old 11-16-2011
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Quote:
Originally Posted by dr.muhamad View Post
no i didnt take them i advice u to take 7 coz 12 was really easy and u dont want to overestimate yourself
actually i got 185 right out of 200 in 12 and i wonder if that is good
but when i was solving 7 it seems a lil bit harder

dont freak out my friend you r going to win this battle just relax and try to be good with your FA if u want any help we can talk on skype or emails
Wow 185/200 sounds pretty good to me. I am sure you will do fine in the exam! when is yours? I will take the 7 then. Thanks for your help!
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Old 11-16-2011
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Quote:
Originally Posted by Glomerulus View Post
Wow 185/200 sounds pretty good to me. I am sure you will do fine in the exam! when is yours? I will take the 7 then. Thanks for your help!
my exam on 26/12

btw u can do all the questions in this website
they r pretty good
like start solve them beside ur study wen u take a break and they will improve ur performance
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