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Old 04-03-2010
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Question Hemophilia De Novo Insertion Sequence?

Two patients with hemophilia A have been found to have disease due to de novo insertion of L1 LINE into exon 14 of factor VIII gene during embryogenesis or maternal oogenesis although L1 LINES are common within human genome almost all those carried by humans today are unable to move. which of the following is a characteristic of a very rare insertion that created hemophilia in these two patients?


A) DNA polymerase delta activity was required to insert LINE sequence in the new site
B) homologous DNA recombination was required to insert LINE sequence in the new site
C) RNA polymerase II activity was required to insert to insert LINE sequence in the new site
D) RNA polymerase III activity was required to insert to insert LINE sequence in the new site
E) transposase activity excised the LINE from its previous location and inserted into the Exon
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Old 04-04-2010
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Default Transposase

I think transposase activity is needed for any insertion, so I go with option E
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Old 04-04-2010
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Question E really?

E are u sure? i hadnt read anything to do with eukaryotic genes and transposase. does the same apply?
doctor F where are u getting these questions? they seem so.. arrgggghhh!!
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Old 11-18-2011
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any answer??
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Old 11-18-2011
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E) transposase activity excised the LINE from its previous location and inserted into the Exon
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Old 12-03-2012
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Default

Quote:
Originally Posted by doctorF View Post
Two patients with hemophilia A have been found to have disease due to de novo insertion of L1 LINE into exon 14 of factor VIII gene during embryogenesis or maternal oogenesis although L1 LINES are common within human genome almost all those carried by humans today are unable to move. which of the following is a characteristic of a very rare insertion that created hemophilia in these two patients?


A) DNA polymerase delta activity was required to insert LINE sequence in the new site
B) homologous DNA recombination was required to insert LINE sequence in the new site
C) RNA polymerase II activity was required to insert to insert LINE sequence in the new site
D) RNA polymerase III activity was required to insert to insert LINE sequence in the new site
E) transposase activity excised the LINE from its previous location and inserted into the Exon
C) RNA polymerase II activity was required to insert to insert LINE sequence in the new site
I think C is more probable then E.
As LINE (long interspreaded nuclear elements) uses copy- paste mechanism to insert there sequence in genome. (So inmovable)
Whereas
Transposons use cut-paste method.

I know its outdated question but looking for insight guys.
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