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Old 04-15-2010
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Default Blotting Technique!

A researcher is trying to identify a specific protein within a mixture. He subjects the mixture to gel electrophoresis and then transfers the separation to nitrocellulose filters. The filters are incubated with antibody to the specific protein and the excess antibody is washed off. The antibody-protein complex is then incubated with a radiolabeled protein that binds to the antibody. Autoradiography is performed to detect the presence of the protein. This technique represents


A. Northern blotting
B. Southern blotting
C. Southwestern blotting
D. Western blotting
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Old 04-15-2010
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Default D ?

is it western blotting?

ive done this technique before but up till u mentioned autoradiography?? ive never use autoradiography to view my western blot membranes. the protein just shows up with the substrate used.

and whats southwestern blotting?
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Old 04-15-2010
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Quote:
Originally Posted by Seetal View Post
is it western blotting?

ive done this technique before but up till u mentioned autoradiography?? ive never use autoradiography to view my western blot membranes. the protein just shows up with the substrate used.

and whats southwestern blotting?

Southwestern blotting involves DNA-protein interactions. A protein sample is subjected to electrophoresis, transferred to a filter, and exposed to labeled DNA
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Old 04-15-2010
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Default

so then its western blotting!!
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Old 04-15-2010
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Default

the ans is western blot.
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Old 04-16-2010
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Yeah.D is correct Western blots are used to identify a specific protein that may be present in very small concentrations in a complex protein mixture. This technique is used to confirm the presence of anti-HIV antibodies in the serum of infected patients
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Old 04-16-2010
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Default Explained further

Have a look here also
Northern vs Southern vs Western Blot - HELP!
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