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Old 06-23-2012
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Default A pharmaceutical firm is interested in the bacterial...

A pharmaceutical firm is interested in the bacterial production of thymidylate synthase in large quantities for drug-targeting studies. An important step in the overall cloning strategy involves the ligation of synthase cDNA into a plasmid vector containing a replication origin, an antibiotic resistance gene and a promoter sequence. Which additional nucleotide sequence should be included in this vector to ensure optimal production of the thymidylate synthase?

a) operator sequence
b) polyA sequence
c) Shine-Dalgarno sequence
d) attenuator sequence
e) 3'-splice acceptor sequence

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  #2  
Old 06-24-2012
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a) operator sequence
b) polyA sequence
c) Shine-Dalgarno sequence binding site for prok.RNA polymerase
d) attenuator sequence
e) 3'-splice acceptor sequence
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Old 06-24-2012
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C. Shine dalgarno sequence
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  #4  
Old 06-26-2012
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Default correct answer

yup, C is the correct answer
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