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Old 10-28-2012
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Default Endotoxin gel-clot test - interpretation

Hi guys,

Id like to get your opinion about an endotoxin test interpretation. At the beginning we must establish our std curve that consists of 4 standards of the following CSE (control standard endotoxin) concentration:

1. 0.25 lambda (0.015 IU/mL)
2. 0.50 lambda
3. 1.00 lambda
4. 2.00 lambda (0.125 IU/mL)

As a sample we have WFI water (conductivity about 1.0 uS/cm @ 25 C).
After the incubation (1h/ 37C) we obtain the following results:

1. - ve
2. + ve
3. + ve
4. + ve AND our sample is - ve.

Hence we can say that endotoxin in the sample is less than the endotoxin concentration corresponding to 2. 0.50 lambda. Now what in case if in ALL 4 standards we obtain the result +ve and the sample is - ve? Can I still have the rights to say that the endotoxin concentration in the sample is less than 0.015 IU/mL? Or rather my test is not valid as the one point in the std curve had to be negative ( 0.25 lambda should be -ve to make the std curve valid) and it was not?

Im just wondering whats your view on that ?
All opinions more than welcomed

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Old 10-28-2012
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is this a UWORLD question? looks tough!
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Old 10-28-2012
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My review
hahaha. Just joking
Never came across this concept. If you don't mind. Can you share more details about this and its interpretation. As not able to get much out of your above mentioned post.
One day i will infect these microbes and they will search for "Anti-MEbiotic"....
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