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Old 05-01-2013
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Help g-csf bioassay issues

we perform bioassay of g-csf using m-nfs-60 cell lines but we do not get reproducible results. some time a sample fails and on repeating the assay for same sample it passes. we do not get proper gradation too. can someone please provide me a protocol for g-csf bioassay that has been successfully used. please help me.
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