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Old 11-07-2010
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Bacteria Chlamydia & its Forms

It's confusing 'cause I saw that question almost on all Qbanks:
On tissue cultures, which form of chlamydia do you see? Isn't it suppoesd to be the reticulate body 'cause its the replicating form??
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Old 11-07-2010
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From qbanks, I've learned that the elementary body is the particle that can be seen in cultures. However, information from the internet shows that both forms can be seen, and in fact, elementary bodies can be seen only within 50 hours after infection, before they morph into reticulate bodies, or when the infected cells rupture, releasing such elementary bodies.

Source: Wikipedia.
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Old 11-07-2010
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The elementary body which is extracellular is the one to see in tissue culture???
Arrgh, thats really confusing!
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In the attached diagram of an idealised chlamydial developmental cycle, The small, infectious elementary bodies are in red; the larger, replicating reticulate bodies are in green.


Chlamydial infection is initiated by attachment of a chlamydial elementary body to the host cell, followed by its entry into the cell.
The chlamydial elementary bodies are internalised in tight, endocyctic vesicles, within which they differentiate into reticulate bodies.

Chlamydia & its Forms-chlamydia-bodies.jpg
Click to enlarge

http://www.chlamydiae.com/docs/biolo...l_devcycle.asp
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Old 11-07-2010
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Quote:
Originally Posted by Zein View Post
The elementary body which is extracellular is the one to see in tissue culture???
Arrgh, thats really confusing!
If you think about it, you'll notice that extracellular bodies/organisms can be seen in cultures more readily than intracellular ones. Examples: rickettsiae (obligate intracellular) & legionella (facilitative intracellular) are not readily visualized on gram stain. The same applies to reticulate bodies.

Source: First Aid for Step 1, 2010 ed. p. 140 (top of page).
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Old 11-07-2010
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Just wanna say have a look here
Nice Microbiology Videos
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Old 11-08-2010
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Thanks for your replies people, I think for exam purposes it's elementary then.
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