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Old 05-25-2011
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USMLEWorld UWSA Question about wich set of primers for the PCR

Can anyone please explain me how PCR reaction works?

I got a question in UWSA, regarding which set of primer can yield complimentary DNA copies? I am not sure if I can post a screen shot of the exact UWSA question over here..

DNA is synthesized in 5 --->3 direction so ucleotides will be added to the 3 side of the strand but this question is not making sense to me.
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Old 05-25-2011
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May be it helps you for start:

Or write here the concrete question... It is also important to know temperature of each step of PCR.

PS. Oh I think I understood what about you asking...
Do you mean why primers add to 3' but target strand synthesized 5' -> 3'? If yes that watch carefully the 1st minute of animation which I sent.
Instead of saying “we don’t know why,” though, say “idiopathic.” It sounds so much smarter.

Last edited by deyneko; 05-25-2011 at 08:04 AM.
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The above post was thanked by:
struggle (05-25-2011)


Genetics-, Molecular-Biology-, USMLEWorld, Videos-Animations

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