Northern vs Southern vs Western Blot - HELP! - USMLE Forums
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Old 11-30-2009
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Default Northern vs Southern vs Western Blot - HELP!

Hello,

I have been getting questions related to Blots wrong =[ I have been going with the mnemonic: "SNoW DRoP":
Southern-DNA
Northern-mRNA
Western-Protein

But, this apparently is not enough to answer the questions correctly. Can someone PLEASE clarify this concept for me and how i can answer questions related to this topic!

THANK YOU!
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Old 11-30-2009
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Try looking at Lippincott biochem..great table that will help you remember the differences between each.

GL.

Last edited by Sabio; 12-21-2009 at 08:37 AM. Reason: Linking text
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Old 12-01-2009
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It all depends at what you want to detect
Looking for DNA, then you need a complementary DNA probe (South)
Looking for RNA, then you need a complementary DNA probe (North)
Looking for Protein, then u need a binding antibody (West)
Looking of DNA binding protein, then u need a DNA probe (Southwest)
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Old 12-21-2009
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Default DNA Binding Proteins = Southwest Blot

Just to add to this
Examples of DNA binding proteins that are commonly tested in USMLE are:
Steroid receptors, Thyroid hormones, Retinoic Acid receptors, and Transcription factors.
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Old 12-21-2009
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Question

I don't get looking for a DNA binding protein??!!

How would the question scenario be?
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Old 12-21-2009
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Quote:
Originally Posted by DrSeddik View Post
I don't get looking for a DNA binding protein??!!

How would the question scenario be?
It would be something like this
" you did cDNA clone and made it to express a product then you used a DNA probe to label it, what product are we looking for?
a- glucagon
b- CFTR receptor
c- lysosomal enzyme
d- Zink finger
e- RAS protein

Although all the options are porteins but only Zink finger protein is a transcription factor that binds DNA and leads to increased gene expression
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Immunology-, Molecular-Biology-

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