A researcher is trying to identify a specific protein within a mixture. He subjects the mixture to gel electrophoresis and then transfers the separation to nitrocellulose filters. The filters are incubated with antibody to the specific protein and the excess antibody is washed off. The antibody-protein complex is then incubated with a radiolabeled protein that binds to the antibody. Autoradiography is performed to detect the presence of the protein. This technique represents
A. Northern blotting
B. Southern blotting
C. Southwestern blotting
D. Western blotting
A. Northern blotting
B. Southern blotting
C. Southwestern blotting
D. Western blotting