[khanar]

TACGATCATAT is an oligonucleotide probe used to construct a cDNA library to study the transcriptome of genetically modified mice.
Which of the following coding strands will correctly hybridize with the probe in question?

A- TACGATCATAT
B- ATATGATCGTA
C- AUGCUAGUAUA
D- GCATATACGCG
E- AUAUGAUCGUA

 

[Aditya]

C

C IS the ans i guess

 

[khanar]

It's not C

C IS the ans i guess

So contains uracil, DNA template strands do not contain Uracil
I think the answer is B

 

[Aditya]

ooops

yea a blunder, i made...havent read mol biochemistry yet..

 

[ashishkabir]

Agreed - i think it's B too...

 

[bianca]

B, cDNA complementery & antiparallel. Match the nucleotide starting from the back, just a hit. It works for me.

 

[khanar]

Yes

B, cDNA complementery & antiparallel. Match the nucleotide starting from the back, just a hit. It works for me.

Yes, you are absolutely right. This is the best way to solve these questions

 

[rah_mem]

Its "B" .....

 

[Seetal]

yep yep B

definitely. thats what i did. cDNA, then find its hybrid and reverse it because the end result (even for primer design) must always be shown in the sense strand (5' to 3') fashion.

 

[ely83]

I think the correct one is answer B:

since they don't specify, you assume that the sequences are written in a 5'-3' direction. The hibridizing (binding) segment to that oligonucleotide should be complementary to it, thus it should be 3'-ATGCTAGTATA-5'. But since they write it on a 5'-3' direction, you have to reverse it. Thus it becomes 5'- ATATGATCGTA-3', answer B.

Does it make sense?

TACGATCATAT is an oligonucleotide probe used to construct a cDNA library to study the transcriptome of genetically modified mice.
Which of the following coding strands will correctly hybridize with the probe in question?

A- TACGATCATAT
B- ATATGATCGTA
C- AUGCUAGUAUA
D- GCATATACGCG
E- AUAUGAUCGUA

 

[Seetal]

yes ely83

I think the correct one is answer B:

since they don't specify, you assume that the sequences are written in a 5'-3' direction. The hibridizing (binding) segment to that oligonucleotide should be complementary to it, thus it should be 3'-ATGCTAGTATA-5'. But since they write it on a 5'-3' direction, you have to reverse it. Thus it becomes 5'- ATATGATCGTA-3', answer B.

Does it make sense?

yes ely. that's what i meant. reverse it!! awesome. we're at par.

 

[drlizard]

does using the template instead of coding changes the answer?