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Hi guys,

Can someone briefly discuss the electrophoresis of Hb A Hb S Hb C , cause i feel I'm not getting the idea of Hb C getting 2 +Ve charge compared to Hb A. and less mobility on electrophoresis!!

I know in Hb S there is substitution of Glutamate acid at position 6 with valine and Hb C the same but with basic lysine.

I feel I'm not understanding the electrophoresis

thanks
 

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A fully protonated Lysine (HbC) has +2 Net charge therefore it's two positive charges extra compared to the neutral valine (HbA) and therefore it migrates further away from the positive electrophoretic pole.

Glutamate (HbS) is acidic) and has -1 Net charge at physiologic PH, therefore HbS is one less negative charge (more positive polarity) and therefore it too shifts away from the positive side of the electrophoresis gel but to a lesser extent than HbC.
 
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