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Northern vs Southern vs Western Blot - HELP!

20145 Views 5 Replies 5 Participants Last post by  Sabio
Hello,

I have been getting questions related to Blots wrong =[ I have been going with the mnemonic: "SNoW DRoP":
Southern-DNA
Northern-mRNA
Western-Protein

But, this apparently is not enough to answer the questions correctly. Can someone PLEASE clarify this concept for me and how i can answer questions related to this topic!

THANK YOU!
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It all depends at what you want to detect
Looking for DNA, then you need a complementary DNA probe (South)
Looking for RNA, then you need a complementary DNA probe (North)
Looking for Protein, then u need a binding antibody (West)
Looking of DNA binding protein, then u need a DNA probe (Southwest)
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