[rsin21]

which of following enzyme function will you test to look for thiamine deficiency??

A : pyruvate hehydrogenase
B : alpha-ketoglutarate dehydrogenase
C : acetyl carboxylase
D : transketolase

 

[drahmednawaz]

D. Transketolase.

Because, it requires only thiamine, while other dehydrogenases require thiamine+other factors as well.

 

[Mondoshawan]

D. Transketolase - because it requires only thiamine, while other dehydrogenases require thiamine + other factors as well.

Definitely!

Now if the question were:
A patient presents with suspected thiamine deficiency; what is the most appropriate diagnostic measure?

• administer thiamine
• assay erythrocyte transketolase activity
• measure serum alphaketoglutarate
• measure serum thiamine
• measure urinary methylglyoxal
• perform thiamine loading test

the best answer would be A. administer thiamine...

 

[rsin21]

Answer is D

Reason (as already told by drahmednawaz)

A&B require cofactors like : thiamine pyrophosphate, lipoic acid, coenzyme A, flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NAD).

C : does not require thiamine

D :requires only thiamine