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Discussion Starter #1
Does topoisomerase I relax supercoils?
Does that mean it causes negative supercoiling of DNA?
and how does that occur?
 

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Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!
 

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Discussion Starter #3
Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!
Thanks Alot. I thas been very helpful.
Best of luck to you too. :)
 

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487 Posts
Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!

 
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