[hope007]

Does topoisomerase I relax supercoils?
Does that mean it causes negative supercoiling of DNA?
and how does that occur?

 

[Myticketout]

Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!

 

[hope007]

Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!

Thanks Alot. I thas been very helpful.
Best of luck to you too.

 

[aktorque]

Hey.
Yes, Toposismorase I relaxes super-coils. It does so by cutting 1 DNA strand.
Topoismorease II also relaxes super-coils but does so by cutting both DNA strands.
When you relax (decrease) a coil it is called Negative super-coiling. (removing stress)
When you increase the coil it is called positive super-coiling.
Tpoisomerase works by cutting the strand, passing it through the other strand and annealing it back.

Hope this is helpful.
Good luck!