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Bacterial Multiplication Calculation

3.2K views 17 replies 9 participants last post by  venky2600  
#1 ·
A flask is inoculated to a density of 3 x 1000 cells/ml. What will be the density of cells in the culture after 50 minutes if the generation time is 20 minutes and the lag time is 10 minutes?
A. Same density
B. Half the density
C. Double the density
D. Four times the density
E. Given values insufficient to determine
 
#4 ·
CC?

CC...I think it should be doubled after 30 minutes since no growth at 10 minutes in lag phase.
But the last 20 mins is confusing?but it should be in stationary phase?
 
#12 ·
C. Double the density ?????



Dude you really know how to test our easy looking but always making confused topics.:D
So thats how i approach question-
Lag time- no particular cell growth

generation time- rapid dividing phase (may bacteria's undergo mitosis. So guess it it be 2 X original density)

but in all together 50 mins we should include stationary and death phase. (but as there will be no particular growth)
So not sure what will be the final answer.

As i didn't did much of reading recently but If you will just not answer question but also discuss your approach. Thanks
 
#14 ·
Dude you really know how to test our easy looking but always making confused topics.:D
So thats how i approach question-
Lag time- no particular cell growth

generation time- rapid dividing phase (may bacteria's undergo mitosis. So guess it it be 2 X original density)

but in all together 50 mins we should include stationary and death phase. (but as there will be no particular growth)
So not sure what will be the final answer.

As i didn't did much of reading recently but If you will just not answer question but also discuss your approach. Thanks
Fren it goes like this, generation time is the time taken by the cells to double which is possible only bye division i.e. only in log phase...............here in total of 50 mins time, lag phase is 10 minutes (lag phase is the one where cells are resting and no. of cells do not change at all)................remaining time is 40 minutes.............if cells double in 20 minutes (in question generation time=20 minutes), then in 40 minutes, it'll double two times i.e. four times the original..................hope this helps............thanx.
 
#16 ·
@subbi---thanks..actually at first i wasn't sure...but after looking at @obinocle approach..i concluded my answer....

@koolkiller88 @neelima208--------adding a point to subbi's explanation.......unless the bacterial growth specifies(like for ex--10000cells/ml is limit),doubling time continues as long as the time given(i mean they will be in log phase)...we should consider the whole time given under doubling time only..no entry to stationary phase too

hope this understands..thanks:)
 
#17 ·
Vencky2600
I don't get it right.pls explain alittle

From lag to log phase and then to stationary and so.
Why do you stop at log phase?
I reasoned it should cont. to remaining phases after a single doubling time??